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Algonquian and Iroquoian tribes.
It created the current borders. The concept of 'nation-States' was (apart from Ethiopia) unknown south of the Sahara. There were only tribal lands with only vaguely defined borders that were continually and often hotly contested between the tribes.
Slavery. Although the Spanish religious doctrine greatly inhibited enslaving indigenous tribes, other European countries soon imported slaves from Africa to operate plantations.
Choctaws, Chickasaws, Creeks, Seminoles, & the Cherokee were the largest civilized tribes.
GothsVandalsFranksBurgundiansAlemanni