Yes. Their leader had to return to England for about three months and when he returned, the settlers he had left were no where to be found.
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The Roanoke Colony had a governor. It was not a religious colony.
Sir Walter Raleigh was the govenor of the lost colony of Roanoke.
Sir Walter Raleigh
The first permanent colony was Jamestown. While it did not prosper in the beginning it was the first colony never to be completely abandoned. During and after the leadership of John Smith things started to look up for the colony, and while it hit a few more major stumbling blocks it eventually became a successful enterprise
Spain was preparing to invade England. Spain and England were in a war and he could not reach his ship so he had to postpone his arrival to Roanoke.