No. It is the border between Maryland and Pennsylvania.
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The "Mason-Dixon" Line Not true, the Mason-Dixon line was used due to colonial disputes with the British colonies at about 1763
The Mason-Dixon line was defined as the line extending west from the border between Pennsylvania and Maryland. As Baltimore is located south of the Pennsylvania border, it is south of the Mason-Dixon line.
Mason and Dixon surveyed the border between Maryland and Penn's domain of Pennsylvania and Delaware State. This tour follows the southern border of Pennsylvania covered bridges in Pennsylvania, Delaware and Maryland. This is a tour of covered bridges, not of the Mason-Dixon line
This answer is written as if looking at the Mason Dixon Line at the beginning of the Civil War. Florida, Georgia, Alabama, Mississippi, Louisiana, Texas South Carolina, North Carolina, Virginia (including the future West Virginia) Maryland Kentucky, Tennessee, Missouri California Technically, the Mason-Dixon line simply established the boundary that cut between Pennsylvania, Maryland, and Delaware. It came to symbolize the cultural divide between slave economies and free economies. At the time the line was drawn, however, slavery was legal in nearly all parts of the country. The Mason-Dixon Line is sometime confused with the line (36 degrees 30 minutes north) of the Missouri Compromise; that line was intended to limit the growth of slavery in the years leading up to the Civil War.
Charles Mason became famous with the Mason-Dixon Line, which was commonly associated with the division between the northern and southern states during the 1800s. The line was to settle a property dispute.