The Mississippi river was the border.
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The Appalachian Mountains became the western boundary of the British colonies.
The Appalachian Mountains provided a natural barrier to the western expansion of the English Colonies in America, or so some thought.
The mountain range that formed the western boundary of the original states was the Appalachian Mountains. The mountains also extend into southeastern Canada.
The southern boundary of the Northwest Territory is the Ohio River, which was provided a means of governing through the Northwest Ordinance of 1787.
Ceded its western claim that extended Mississippi River to the Federal Government.