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Yes and No. One part of the series of laws was the Boston Port Act which did close Boston Harbor which was a key trade port in the colonies and one of the biggest in New England, so it did cut off trade from New England, and essentialy the north, from the south. It did not cut off trade between the American colonies and England the country.
The intolerable Act
The United States declared its independence on July 4, 1776. This was when the 13 colonies cut off all political affiliations with Britain.
because they needed to regulate trade and help pay for the troops off fighting
In 1777, the British' attempt to cut New England off from the rest of the colonies failed when Burgoyne surrendered his army at Saratoga.