Yes. Franklin Delano Roosevelt became President in 1933, and was re-elected for a 4th term in 1944. He died April 12, 1945, about 3 months after being sworn in for his 4th term.
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Harry S. Truman followed FDR after the latter's death in office .
Their choice of vice-president would take office as the new president if the president-elect should die.
The 9th President of the United States William Henry Harrison was the first to die in office in 1841 only 32 days into his term.
FDR
Yes ,William Henry Harrison was the first president to die while in office. He died in the White House after catching a cold.