No, he did not. When Perseus met Atlas he had already seen the three sisters and killed Medusa.
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Because Atlas remembered an ancient prophecy which had warned him that a son of Jupiter [which is Perseus] would one day rob him of his golden apples.
He needed to ask them where the nymphs of the north lived because he needed 3 magical items from them. When he got their he took their only eye and forced them to tell him where they lived.
To force them to tell him where Medusa, the gorgon lived.
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As a fact, we can never know and the only people who could tell us are the historians. Seeing whom Poseidon's rivals are though, Athena, Zeus and Hades' are the ones who would DISLIKE Perseus and they're about the closest to RIVALA.