Due to the ferocity of the fighting and destruction and chaos of the war, and the fact that neither side keep very accurate records it is impossible to say for sure. The best estimate is about 500,000 killed from all causes.
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Massive.
If the question is asking about the Civil War battle with the highest death toll, I believe it was the Battle of Antietam.
The war ended in 1918. That coincided with the outbreak of Spanish Influenza. The outbreak turned into an epidemic. The death toll of both soldiers and civilians increased drastically because of the disease.
While it is not known for sure, the total would be in the tens of thousands. About two thirds of the deaths in the Civil War was due to disease including gangrene.
the spanish civil war occured between 1936- 1939