One possible reason that a discharge petition is seldom successful is because so many signatures are needed on the discharge motion.
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they ran out of food and weapons
Worldwide demand for cotton
They did so to reaffirm the colonists' allegiance to the king rather than Parliament.
The failure of reconstruction is universally agreed on, but the exact reason is a source of debate. The slaves were freed, but racism and hatred continued. The government did not give the slaves the tools to become successful.
the main reason to do immunodiffusion test is to quantify antigen or antibody