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the main reason to do immunodiffusion test is to quantify antigen or antibody

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How does immunodiffusion assay differ from immunoelectrophoresis assay?

Immunodiffusion assay is based on the diffusion of antigens and antibodies through a gel medium, allowing the formation of a visible line of precipitation when they interact. Immunoelectrophoresis assay combines electrophoresis to separate protein antigens and antibodies followed by immunodiffusion to determine the specificity and quantity of the proteins based on the bands of precipitation formed. In summary, immunoelectrophoresis is a more complex technique that uses both electrophoresis and immunodiffusion principles to analyze proteins while immunodiffusion is a simpler method based solely on diffusion.


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